New advancements made against drug resistant germs

22 Nov New advancements made against drug resistant germs

Scientists have developed a simple way of altering antibiotics that could make them much more
powerful against infections caused by drug-resistant microbes. The method made the antibiotic
vancomycin much more powerful against two strains of bacteria that had become drug-resistant.
The researchers suggest that the simple chemistry involved in modifying the drug can be applied
to other antibiotics and even to anticancer drugs. A paper on the "bioconjugation technique" now
features in the journal Nature Chemistry.
He and others at MIT worked on the study with colleagues from Yale University in New Haven,
CT, and the biotech company Visterra, which is also based in Cambridge, MA.
Antibiotic resistance develops because every time someone uses an antibiotic drug, a small
population of naturally resistant microbes survives. Resistance spreads not only because the
resistant germs predominate, but also because they share their resistance with other microbes.
A global review published in 2016 suggested that around 10 million lives per year could be at
risk due to increasing antimicrobial resistance.
Without effective antibiotics, many medical procedures — including cesarean delivery, bowel
surgery, chemotherapy, and joint replacement — could pose such a high risk of infection that
they "become too dangerous" to carry out.
Every year in the United States, antibiotic-resistant germs infect around 2 million people and are
responsible for around 23,000 deaths, according to the Centers for Disease Control and
Prevention (CDC).
Infections due to germs that have become resistant to antibiotics are very difficult, and in some
cases impossible, to treat. Alternative treatments tend to cost more and have worse side effects.
People with these infections often have to stay in the hospital longer and need more visits from
the doctor.
The chemistry of the new method came from a previous discovery that the amino acid
selenocysteine can act as a "handle" for attaching small-molecule drugs, such as vancomycin, to
small proteins called antimicrobial peptides, which form part of the immune defenses of most
organisms.
When they used the method to attach the peptides to vancomycin, the scientists found that they
consistently attached to the same place on the antibiotic, producing molecules that were
chemically identical.
Existing approaches to making such molecules would not be able to achieve such purity and
would need more than a dozen steps to prepare vancomycin for attaching to peptides, note the
researchers.

They tested several "conjugates" of vancomycin paired with various different antimicrobial
peptides, including one called dermaseptin. The tests showed that combining vancomycin with
dermaseptin made the antibiotic five times more potent against the infectious bacterium
Enterococcus faecalis.
E. faecalis is a strain of Enterococcus, a genus of bacteria that has emerged as an important cause
of infection in healthcare settings. Urinary tract infections are the most common types of
infection that these bacteria cause, and the "rapid rise" of their resistance to vancomycin has been
a particular worry to medical professionals.
In addition, the team found that vancomycin combined with another antimicrobial peptide called
RP-1 killed Acinetobacter baumannii, against which vancomycin alone has no effect. A.
baumannii is also highly drug-resistant and a frequent cause of healthcare-associated infections.
Tests with around 30 other molecules, including resveratrol and serotonin, suggest that the
approach can easily link peptides to practically any organic molecule that has the "right kind of
electron-rich ring," note the researchers.
However, they point out that they did not test the safety of the modified drugs.
They suggest that their method could also apply to other types of drug; for example, to attach
antibodies to anticancer drugs so that they reach particular targets without harming healthy
tissue.
The authors conclude, "Given these results, we believe our chemistry will be a valuable
bioconjugation technique for the community and may lead to the generation of therapeutic
conjugate molecules."

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